N Y Gbandjaba
Université de Sherbrooke, Canada
Title: Oxidative stress biomarkers measurements, particularly Paraoxonase 1 phenotype distribution is associated with cardiovascular diseases risk in healthy, diabetic and hemodialysis patients
Biography
Biography: N Y Gbandjaba
Abstract
Aim: The aim of the study was to investigate the Paraoxonase 1 (PON1) phenotype distribution and to measure oxidative stress markers (Vitamin E/CT, MDA) in healthy, diabetic and hemodialysis patients. Patients and Method: Three hundred subjects (healthy, diabetic and chronic renal failure patients) aged between 40 and 80 years were recruited for the study (divided in three groups of 100 subjects each). Total MDA content in plasma was measured by HPLC with thiobarbituric acid (TBA) and with fluorescence detection. Vitamin E (α-tocopherol) as the principal plasma antioxidant was also measured by HPLC with electrochemical detection. Results: Vitamin E-Tot. Cholesterol ratio increases significantly with age in diabetic patients (r= 0.30; p <0.01). Vitamin E - Total Cholesterol ratio decreases significantly with age in hemodialysis patients (r= ― 0.17; p<0.05) and in healthy subjects (r=―0.15; p=0.44). Plasma MDA concentration increases with age in diabetic patients (r= 0.09; p=0.15). The distribution of PON 1 phenotype in healthy, diabetic and hemodialysis patients was as follows: AA 79.31%, 75.00% and 69.31%; AB 14.94%, 19.04% and 20.45%; BB 5.74%, 5.95% and 10.22%. Adjusted odds ratio comparing the AA variant with the BB variant were 1.97 [95 % confidence interval (CI): 0.63-6.21] in hemodialysis patients. In diabetic patients, the adjusted odds ratio comparing the AA variant with the AB variant of PON1 were 1.37 [95% CI: 0.62-3.04]. Conclusion: Our studies show that PON1 phenotype distribution and oxidative stress markers are associated with cardiovascular diseases risk in diabetic and hemodialysis patients.