Ivane Javakhishvili Tbilisi State University, Georgia
Title: New nanoscale fluorescent method for analysis of quality of DNA double helix
Biography: T G Giorgadze
The goal of this work is using the method of laser - induced fluorescence resonance energy transfer (FRET) of electronic excitation in the donor-acceptor pair of intercalators, (acridine orange as a donor and ethidium bromide as an acceptor), for quantitative analysis of the quality of DNA double helix. The approach allows getting a visual picture of the defects of genetic apparatus of tissue cells, particularly of skin cells in real time and it can be used for diagnosis of skin diseases, also in cosmetology.
Transition metal ions such as Cu(II), Cu(I), Ag(I), silver nanoparticles (AgNPs), photo- and thermo effects were used to cause double helix defects in DNA.
Concentration of DNA sites, which are applicable for intercalation, after the exposure to Cu(II), Cu(I), Ag(I) ions, AgNPs impact as well as laser irradiation (λ = 457 nm) and temperature, which are applicable for intercalation, were estimated in relative units.
The nanoscale FRET method allows estimation of the concentration of double helix areas with high quality stability applicable for intercalation in DNA after it was subjected to stress effect. It gives the opportunity to compare DNA-s of 1) different origin; 2) with various damage degrees; 3) being in various functional state.